Composite

Part:BBa_K2272019:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-25)


pVHb-HAO-Cyt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 484
    Illegal XhoI site found at 2048
    Illegal XhoI site found at 4257
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 500
    Illegal NgoMIV site found at 977
    Illegal NgoMIV site found at 981
    Illegal NgoMIV site found at 2749
    Illegal NgoMIV site found at 3139
    Illegal NgoMIV site found at 3406
    Illegal NgoMIV site found at 3454
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 404
    Illegal BsaI.rc site found at 452
    Illegal BsaI.rc site found at 1019
    Illegal BsaI.rc site found at 3179
    Illegal BsaI.rc site found at 4856
    Illegal BsaI.rc site found at 5012


Design Notes

pVHb was compared to pBAD with this same construct.


Source

BBa_K2272000, BBa_K2272002, BBa_K2272003

References